50th Regiment or 50th Infantry Regiment may refer to:
- 50th Anti-aircraft Missiles Regiment (Romania), a unit of the Romanian Army
- 50th (Queen's Own) Regiment of Foot, a unit of the United Kingdom Army
- Shirley's Regiment (50th Regiment of Foot, American Provincials), a unit of the United Kingdom Army
- 50th Royal Tank Regiment, a unit of the United Kingdom Army
- 50th Infantry Regiment (United States), a unit of the United States Army
- American Civil War regiments
- Union (Northern) regiments
- Confederate (Southern) regiments
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